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For analysis of cells embedded in soft agar, cells (10 5 ) were suspended in 1 mL of 0.33% noble agar, seeded in the bottom of 15-mL polypropylene tubes, cooled for 5 min on ice, and then kept at 37 C in a humidified incubator in the presence of 5% CO 2 for 24 h72 h

doi: 10.5114/pg.2020.95554 Damianos JA, Fredrick TW, Jin MF, Ospina-Velasquez L, Wang XJ

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Aim for at least 8-10 glasses per day (or more if youre sweating it out in a sauna)